Re: [DIYbio] Re: Non-Platinum Electrophoresis Electrodes That Don't Degrade

On 05/24/2018 11:05 AM, Bio Logis wrote:
> On Wed, May 23, 2018 at 9:05 AM, Bryan Jones <bryan...@gmail.com <javascript:>> wrote:
>
> Interesting find. Conductive polymers might be an option. However, the threshold for conductivity to be a good wire is much
> higher than to dissipate static.

There are conductive carbon based inks for making circuit traces that survive normal circuit processing which includes
washing. They might be water proof enough to last some number of cycles of gel electrophoresis... and not release metal ions...

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Re: [DIYbio] Re: Non-Platinum Electrophoresis Electrodes That Don't Degrade

Hi Abizar,

do you think that 96,0 platinum wire would be ok, or would you recommend 99,9% Platinum?

Do you think that 0,075mm platinum wire would work as well?

Thank you by the way for the tip about NiCr-Electrodes, they seem to work ok.


Am Mittwoch, 23. Mai 2018 18:52:31 UTC+2 schrieb Abizar Lakdawalla:
I used mixed wires, Platinum for one and nichrome (from an old toaster) for the other electrode. So I guess halves the cost. Pt wire can be bought from Jewelry supply houses.
Link to the youtube video describing the construction

On Wed, May 23, 2018 at 9:05 AM, Bryan Jones <bryan...@gmail.com> wrote:
Interesting find. Conductive polymers might be an option. However, the threshold for conductivity to be a good wire is much higher than to dissipate static. If I understand the units correctly (I don't quite get sheet resistance, especially since these are not sheets but bristles), I think these brushes have a conductivity somewhere between that of wood and glass. That is conductive enough to not build up static, but still more of an insulator than a conductor. Are there more conductive polymers out there? I think you'd want something sub-ohm, while these are 10,000-1,000,000 ohms/square. What about something like this: https://www.alibaba.com/product-detail/graphite-polymer-super-thin-high-thermal_60530140212.html?spm=a2700.7724838.2017115.1.22c14c640y8th5&s=p?

On Wed, May 23, 2018 at 1:24 AM Björn Johansson <bjor...@gmail.com> wrote:
Hi all and sorry for resurrecting this old thread.

I have retrofitted some old electrophoresis units with stainless steel wire for electrodes and this works using TAE buffer for a while before the 
buffer gets an odd color (green).

However, I recently cam across a superior buffer containing LiAc and boric acid and this seems much more aggressive, the unit fills up with rust after one run.

Platinum wire is an obvious choice, but it is quite expensive. 

I came across something I think wasn't mentioned here: conductive brush wire (sold here).

This wire is probably used for brushes that can not be allowed to build up static electricity.

This wire seems to be made from some plastic with a carbon filler.

Could this be an option?

/bjorn




On Sunday, May 26, 2013 at 7:29:19 PM UTC+1, Josiah Zayner wrote:
I have been testing out a bunch of different metals and they either oxidize to death on the minutes timescale or in the case of stainless steel turn the buffer orange/brown.

Platinum wire is pretty expensive ~$1.00 cm^-1. Has anyone had luck with a cheaper metal at 100V or greater with TAE?

I have tried:
Nickel
Stainless Steel 430
Tinned Copper

Thinking of trying Titanium or Nickel Titanium (<= $4 meter^-1). Anyone ever tried these?


Thanks,
     Josiah Zayner


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