Re: [DIYbio] Paralens fluorescence microscope objective for Hoechst and DAPI?

Abizar,

Thank you - yes I'd be very interested in trying the setup you suggest - please can you post an image? I have Zeiss Standard 25 binocular microscope. The key thing is that I need a sharp fluorescence image with a 100x oil objective.

- Is the setup you suggest compatible with this type of objective?
- Where can I get the required parts and how do I make them fit my microscope (LED, filters, camera)?
- Can I acquire LED lighting that will provide for UV illumination (for use with DAPI, Hoechst)?

Roninlaw,

The price of the core Paralens Advance system with a single objective lens type is approximately £900 including VAT.

Thanks guys,

Mike

On Friday, June 23, 2017 at 11:15:21 PM UTC+1, Roninlaw wrote:
Price?

On Fri, Jun 23, 2017 at 2:25 PM, Mike <alanmb...@gmail.com> wrote:
Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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Re: [DIYbio] Paralens fluorescence microscope objective for Hoechst and DAPI?

An alternative is to place your illuminating led and filter on one eyepiece of a binocular head. And a filter and camera on the other eyepiece. The led beam is semi focused on the specimen through the objective. Fluorescence light goes up the optical path and is divided equally into the eyepiece though only the camera sees the fluorescence image.
Can send you an image of this setup if it helps.

On Jun 23, 2017 2:25 PM, "Mike" <alanmbeattie@gmail.com> wrote:
Hello all,

As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.

(Please see page 9 of the PDF for an illustration of light spectra)

The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). [b]Question[/b]: Will this device work with Hoechst 33258 and DAPI stain (spectra as below)?

- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461

Thank you,

Mike

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