Re: [DIYbio] Gel Red questions



On Tuesday, March 12, 2013 at 12:03:42 PM UTC-7, Nathan McCorkle wrote:
Yeah, that's why big companies and universities don't switch.

EtBr is molecularly known (no one really knows exactly what the gelRed
and sybrSafe molecules are, we know what classes they're in or one
that was described in one patent, etc)

I'm pretty sure that this information cannot be kept secret.  To obtain a chemistry patent, and to publish an accurate MSDS, the structure of the novel active compound must be known and disclosed.

I remember that SybrSafe is in the cyanine dye family, a descendant of Thiazole Orange.  If you look at the Wikipedia article for SybrSafe, a structure is published, and it sure looks like a cyanine dye to me.

https://en.wikipedia.org/wiki/SYBR_Safe
 
Wikipedia entries are also available for GelGreen and GelRed.  They look like acridine orange and ethidium dimers, respectively, with a peptide-ether linker between the individual fluorophores.

https://en.wikipedia.org/wiki/GelGreen
https://en.wikipedia.org/wiki/GelRed

The idea of making ethidium dimers is not new.  An ethidium dimer was available in 1990.  It's still available.  The linker differs from the GelRed linker, but otherwise the two molecules are quite similar.

https://www.thermofisher.com/order/catalog/product/E1169


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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Dennis said it perfectly on all 3 counts.

Ujjwal I thought you were going to develop lower cost PCR. What has stopped your efforts from succeeding?

-Josh


-Josh

On Fri, Apr 21, 2017 at 8:47 AM, Dennis Oleksyuk <dennis@oleksyuk.me> wrote:
Because building hardware is harder than building software. It requires more skills, time, and money. That's the main reason which applies to hardware development as a whole.

Because the number of customers who buy scientific equipment is small. Therefore the manufacturer has to divide the development cost between a smaller number of customers.

Because the cost of consumables and labor for solving a particular problem is usually higher than the cost of the machine. Therefore the buyers are more interested in saving in labor and consumable cost rather than hardware prices.


On Fri, Apr 21, 2017 at 8:42 AM Gerald Trost <gerald.trost@mail.com> wrote:

this is only my honest opinion from my experience:

I bought a open source 3d printer - in industry they had
such things for 3 decades - but they costed
several hundred thousands

the open source thing works fine but for 90% of the time
its in maintenance and I am the machine engineer

I think the open source things are not yet reliable enough.
 
Sent: Friday, April 21, 2017 at 1:57 PM
From: "Ujjwal Thaakar" <ujjwalthaakar@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
How do the economics work out and why have we not seen bigger companies bring down the process in the advent of open source equipment as well as new startups building low-cost equipment?

 

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