The spectrogram here:
implies that the absorbance is almost asymptotic approaching 200 nm.
I got the impression from the web page that HPLC was usually done
with a spectrometer, so the answer to your question would be no. It is
not new, it is a fairly common use. But a DIY automated HPLC device
might be new.
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Get a free science project every week! "http://scitoys.com/newsletter.html"On Wed, Nov 30, 2011 at 9:10 AM, John Griessen <john@industromatic.com> wrote:
On 11/29/2011 07:24 PM, Simon Quellen Field wrote:"Methanol, for example, absorbs at wavelengths below 205 nm, and water below 190 nm.
Once you have an automated spectrograph, here is something you
might consider using it for:
"http://www.chemguide.co.uk/analysis/chromatography/hplc.html"
If you were using a methanol-water mixture as the solvent, you would therefore have
to use a wavelength greater than 205 nm to avoid false readings from the solvent."
They only mention one wavelength. Is that typical? Would a spectrometer
looking through the HPLC column output be something new?
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