I've regularly done something similar for western/immunoblots. The 'protocol' couldn't be simpler, drain antibody into a falcon and put in freezer. Next time you want to use it, thaw and place on membrane.
I've no idea if a similarly basic protocol would work for your application though.
On 31 January 2012 15:03, Cathal Garvey <cathalgarvey@gmail.com> wrote:
Oh, nice idea. Protein recycling; that's one step ahead of the curve!
If the antibody was purified using some peptide-based method, such as
maltose-binding proteins or his-tags, you could try using the same
methods to separate from the finished experiment?
www.indiebiotech.com
On 18/01/12 16:48, Andrea J Bae wrote:
> Hello Everyone!
> I'm an undergrad at Wellesley College starting immunocytochemistry.
> I'd like to know if there are ways to recycle antibodies for ICC. Does
> anybody have any suggestions from experience or any protocol
> suggestions from the literature?
>
> Thanks!
> Andrea
>
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