Re: [DIYbio] Re: Artificial Jellyfish

On Tue, Jul 31, 2012 at 4:59 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> IMHO, Sugar would make a poor substrate for cell culture. The osmotic
> pressure of growing on sugar would be huge; that's one reason why it's
> traditional to add so much sugar to preserves like jam.
>

Cathal, they don't use the sugar to grow on, they print a mold with
the sugar, then add gelatin + cells and pour it over the mold. Once
the gelatin hardens, the mass is rinsed in an orbital shaker with
media or buffer (not sure which) to dissolve the sugar, then that
liquid is replaced with media. In Jordan's case, the mold looks like
vasculature to aid perfusion later (he perfuses with orbital shaking,
but I think he's also working on using a pump driven system).

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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