Re: [DIYbio] electrophoresis... path length design strategies

On 01/31/2013 09:46 AM, Dakota Hamill wrote:
> Let's say we have a piece of filter paper 10cm long, 1 cm wide, and 0.1 cm thick.
>
> Is it wrong to think it could handle similar voltages and currents that an agarose gel could?
No, that sounds plausible/possible.

> Say it was running at 100V and 0.050A. Could you then use V=IR to calculate the resistance of the buffer soaked paper would be
> 2000 Ohms?
>
> Or, via P=IV, P = (0.050A) x (100V) = 5W

Yep. That's plausible/possible. A 5Watt rated resistor is only 1 com long and .4 cm diameter
and it can get warm to hot at 5W, but not char to black. A 10 cm long strip 1cm wide might
be barely perceptibly warm in still air while dissipating 5W since its surface areas are so
much greater. Your guess at resistance could be way off, since gel boxes aren't so skinny
as you described. What if The 5 Watt level happened at a current of .005Amp? then the
Volts would need to be 1KV. I think that might be more normal for a 10X long as it is wide
gel or paper/electrolyte.

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