Re: [DIYbio] Re: Can a plasmid contain antibiotic synthesis and resistance genes?

If the toxin and antitoxin are expressed in the chloroplast, and you export only the toxin, would that not kill the cell? The toxin in question can kill yeast, could probably do it to plants too?

Well, e.g. kanamycin is just toxic to plant cells because it destroys their chloroplasts. (It Inhibits the bacterial translational machinery of plastids) .

But IIRC, it is harmless for eukaryotic ribosomes.



The toxin however, would probably have som toxicity to the plant's nuclear machinery. But maybe it would be too weak to kill it entirely. Or the antitioxin would have to be released into the cytosol too, not having a chloroplast import protein thus not invading other chloroplasts?

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