Re: [DIYbio] Re: Can a plasmid contain antibiotic synthesis and resistance genes?

On Thu, Jan 31, 2013 at 8:53 AM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> Not necessarily; does the toxin enter other cells? Does it get degraded
> quickly by extracellular proteases?
>
> Toxin/antitoxin systems more often appear to be selfish plasmid
> strategies to force individual cells to keep the plasmid. Antibiotics,
> on the other hand, are usually a cellular strategy (which might or might
> not be plasmid-encoded) to kill off the competition.

Well looking through some papers, it seems that plastid transformation
is often selected with spectinomycin resistance genes, where the
spectinomycin is applied externally. So it must be around the main
cell before getting to the plastid for degradation.

It seems theoretically possible that you could just use this 18kb
spectinomycin operon, which is biosynthesis and resistance
http://www.ncbi.nlm.nih.gov/nuccore/EU255259

Found via
The Gene Cluster for Spectinomycin Biosynthesis and the
Aminoglycoside-Resistance Function of spcM in Streptomyces spectabilis
Kyoung-Rok Kim, Tae-Jong Kim, Joo-Won Suh
http://home.kookmin.ac.kr/~bior/Curr.%20Microbiol%2057-371.pdf

--
-Nathan

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