Re: [DIYbio] Re: Can a plasmid contain antibiotic synthesis and resistance genes?

On Thu, Jan 31, 2013 at 12:04 PM, Cathal Garvey (Phone)
<cathalgarvey@cathalgarvey.me> wrote:
> If the toxin and antitoxin are expressed in the chloroplast, and you export
> only the toxin, would that not kill the cell? The toxin in question can kill
> yeast, could probably do it to plants too?
>

Hmm, you could say the same thing about added antibiotic too then, I
think... if the chloroplast is resistant, but the main cell isn't.
Maybe there are some toxins/antibiotics that only affect
bacteria/chloroplast? Or maybe if a plant cell's chloroplast died, it
would simply die as a result? If plant cells live in defined media in
dark conditions, that might tell us if killing/halting just the
chloroplast might be enough.

--
-Nathan

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