[DIYbio] Medium-term storage of bacteria

Hi everyone,


I have got bacteria that carry a plasmid that encodes resistance ageainst tetracyclin.

They are on Tetracyclin-Agar plates. Can I just store them in the fridge for a few months?


I mean, I once stored Ampicillin resistant E.Coli on Amp plates for (don't remember the exact number 3-5 months). Then re-plated it on fresh LB Amp agar, and they grew without problems.

However tetracyclin has to be actively transported out of cells. Now that they were metabolically inactive, the tetracyclin has entered the cell by osmosis.
When they "wake up" again, they probably won't be able to pump it out again, right?

Other theory: It works bacteriostatic, that means, only when cells divide they get killed. So the bacteria resume metabolic activity, and pump out the antibiotic. Then they divide without any problems...


Anyone experience with this?

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