Re: [DIYbio] DNA quantification instruments

I recently bought an old Eppendorf Biophotometer. I have access to a
Nanodrop at my university's campus and the Biophotometer reports DNA
concentrations within one 1 or 2 ng/uL of what the Nanodrop reports.
There are a few downsides to the Biophotometer, though.

1) It only reads at fixed wavelengths (230,260,280 and a few others)
so you can't get a full spectrum measurement like on the Nanodrop.

2) By itself it does not handle microliter volumes. It takes a
cuvette like usual. But it can also handle the Hellma TrayCell or the
Eppendorf uCuvette G1.0. Those are sold separately but allow for
using 1-2uL samples. These devices fit into the cuvette receptacle
and focus the light through a small point where the sample sits. I
have a Hellma TrayCell and it works just fine. It's a little more of
a pain to clean out the old sample compared to the Nanodrop but I
don't mind.

3) It doesn't report data to a computer so you have to resort to
recording your data into your notebook with a pen. I don't mind but I
know this would be a deal breaker for some of my colleagues.

4) The TrayCells are hard to find (occasionally one will show up on
ebay) and the uCuvette G1.0 costs around ~2,000 USD.

The positives are

1) Way cheaper than a Nanodrop.

2) Works just as well as a Nanodrop.

3) Can also do OD measurements so you don't need a separate device.

-cory

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