[DIYbio] Re: Will this sequence work as promotor?

 Extremely hard to say... I just know from T7 if you space lacO (or was it tetO) more than 5 bp away it doesn't work anymore because the DNA helix. The repressor will bind the operator but in a different angle... Have you made sure that the core promoter will be in the same position relative to the operator? Isn't it possible to take the native coli one?

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