[DIYbio] Re: Power tool analogue in MolBio.

What exactly do you want it to do? There are leucine zippers or TALENS or CRISPR, inteins and GS linkers, his tags or FLAG tags, biotin and streptavidin, introns or ribozymes... Each has a specific purpose. And for the termini, yes most likely, unless you want to go through some protein engineering and structure analysis to see if it would fold correctly.

Maybe this will be a lead for the inteins, if you just want a specific protein to fuse to something modularly - http://2014.igem.org/Team:Heidelberg/Parts .

-Koeng
On Saturday, October 31, 2015 at 12:13:18 PM UTC-7, Yuriy wrote:

Hello everyone.


I'll try to be as brief as possible, given the lack of knowledge on the subject. I don't know where to start. That's why I came here with the question.


I was looking at my drill and it dawned on me that there isn't anything like it, to my knowledge, in molecular biology. It is a tool that alone has no use but given the proper fittings and combination of tool:fittings (adapters), can get the job done. The drill bit end for example, has six sides that accommodate the adjustable chuck. That's what I was looking into for the last few days.


Is there a good, on both transcribed, antibody to antigen fitting to adapt fitting to a molecular tool?


If yes do they have to be on termini? Or can it jet out of aa design via linkers?


I essentially need the tool that will be stable in vivo, while the adapters can undergo a higher turnover under different environmental ques.


Just looking for a good non-bulky-lock and key to be encoded for the tool set.


-Yuriy

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