Yeah, don't know if I am interested in selling a SLiCE mix because I still don't know how efficient it is in a DIY setting. That is why I am interested in the results you all are having. And what you all are using, i.e. homemade competent cells vs. high efficiency competent cells, &c. Is just some blunt end ligation occurring or is it an actually legit Gibson like assembly? I will test it out in a few weeks.
Patents don't really hold me back from biology stuff, maybe they should? They say the patent applies to every RecA- bacterial strain but have only tested 5-10? The patent seems flimsy with so many ways around it. Just don't list the bacteria I use? or Use a bacterial strain isolated from my skin that 16s is not an identical match for any known bacteria. They seem like a small company that probably doesn't have enough money to sue and would probably gain very little in damages. But who knows.On Fri, Oct 30, 2015 at 1:22 PM, Bryan Jones <bryanjjones@gmail.com> wrote:
I'm not an expert on reading patents, but it looks like the patent covers the method of the cloning procedure, not the production of SLiCE extract, nor the composition matter of SLiCE extract. Selling the SLiCE mix might not actually violate the patent (that'd be up to the user to do or not do). It also looks like the the patent only covers assembly of fragments that have "20 bp to 52 bp that are homologous". So using 19bp or 53bp homologous regions might be a way around the patent. Maybe a bit less efficient, but it looks like that would still work.--On Fri, Oct 30, 2015 at 3:03 PM Koeng <koeng101@gmail.com> wrote:You received this message because you are subscribed to the Google Groups "DIYbio" group.Yea, my apologies for the non-controlled experiment, I didn't set it up to be very controlled because I didn't think I'd get that good of efficiency. I will be running a few more in the next few weeks so I will post the results.Unfortunately enough, it appears that SLiCE is patented ( http://www.google.com/patents/US20130045508 ), and the company that owns the patent charges an arm and a leg ( http://slicecloning.com/SLiCE.html , more than HiFi mix per reaction). I guess the ODIN can't sell the mix, but possibly sell a kit to make it yourself.-Koeng
On Thursday, October 29, 2015 at 3:12:01 PM UTC-7, Josiah Zayner wrote:JosiahThanks,This is pretty cool. If people try and repeat these experiments can they post detailed details(heh) of what they did so others can follow suite.Also, it doesn't seem anyone is running the control of everything but the SLiCE mixture. If you are running an experiment can you do that and let us know how that goes?On Thu, Oct 29, 2015 at 2:44 PM, Koeng <koen...@gmail.com> wrote:I tried out my homemade SLiCE mix. I put 100ng of both insert and vector in for a 2 piece assembly, for 30 minutes. I compared it to some homemade Gibson mix.
On my plates, I got 13 colonies on the SLiCE plate and 42 colonies on the Gibson. I am extremely impressed with 1/3 considering just the simple strain and simple lysis procedure.
-Koeng
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