Identification of exactly what proteins (with references) confer to this resistance is the first step.
-- Since biological systems for the most part are not modular between different domains (lots of exceptions), you will have to verify that these do, indeed, confer to resistance to radiation. What will your controls be for that? How will you prove statistical significance in mice? Are all proteins needed, some, or just 1?
I agree with Dennis, it will probably not work first try around. Perhaps it will with extreme modification, but that is out of the realm of DIYbio.
-Koeng
On Thursday, January 28, 2016 at 3:59:23 PM UTC-8, Finn Daffron wrote:
On Thursday, January 28, 2016 at 3:59:23 PM UTC-8, Finn Daffron wrote:
Thank you for your help and the link. I have, based on information provided in the paper and by you, determined a method that does not involve live thermococcus gammatolerans but instead the recombinant proteins that provide DNA repair in thermococcus gammatolerans.
On Thursday, January 28, 2016 at 3:11:22 PM UTC-5, Finn Daffron wrote:I am trying to create a batch of thermococcus gammatolerans. This is because I want to use CRISPR to inject thermococcus gammatolerans into rats in order to create radiation resistance with possible implications being people working in nuclear power plants, astronauts, etc. Does anyone have any ideas on how it would be synthesized? Please get back to me.
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