My failed pVIB E. coli experiment has been bugging me and I'm wondering if anyone has any ideas as to why. I got the pre-transformed kit from The Odin which was simple enough. My initial kit did not glow out of the box so Josiah was kind enough to send another one which I verified was glowing. I made additional LB plates and streaked them using the original glowing plate.
-- Easy enough right? Except none of my plates glowed. Zero. I tried different temps, giving them more time to grow, etc. There was also no visible contamination and the cultures appeared to be growing fine. What could be the cause of the lack of glow?
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