Re: [DIYbio] E. coli with pVIB not glowing, why?

I transformed it at university 4-5 years ago. Replated it on LB without Amp lots of times and it got less bright (plasmid loss), but even after 6 subcultures it was still possible to see with the naked eye. Then I left the Amp culture in the Uni fridge over the summer holidays, and after like 3 month put it on fresh LB Amp - glowed again.

So from my experience pVIB worked fine. I don't know which exact coli strain we had at Uni, they were commercial ones

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