Re: [DIYbio] Gel Red questions



On Tuesday, March 12, 2013 at 12:03:42 PM UTC-7, Nathan McCorkle wrote:
Yeah, that's why big companies and universities don't switch.

EtBr is molecularly known (no one really knows exactly what the gelRed
and sybrSafe molecules are, we know what classes they're in or one
that was described in one patent, etc)

I'm pretty sure that this information cannot be kept secret.  To obtain a chemistry patent, and to publish an accurate MSDS, the structure of the novel active compound must be known and disclosed.

I remember that SybrSafe is in the cyanine dye family, a descendant of Thiazole Orange.  If you look at the Wikipedia article for SybrSafe, a structure is published, and it sure looks like a cyanine dye to me.

https://en.wikipedia.org/wiki/SYBR_Safe
 
Wikipedia entries are also available for GelGreen and GelRed.  They look like acridine orange and ethidium dimers, respectively, with a peptide-ether linker between the individual fluorophores.

https://en.wikipedia.org/wiki/GelGreen
https://en.wikipedia.org/wiki/GelRed

The idea of making ethidium dimers is not new.  An ethidium dimer was available in 1990.  It's still available.  The linker differs from the GelRed linker, but otherwise the two molecules are quite similar.

https://www.thermofisher.com/order/catalog/product/E1169


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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Dennis said it perfectly on all 3 counts.

Ujjwal I thought you were going to develop lower cost PCR. What has stopped your efforts from succeeding?

-Josh


-Josh

On Fri, Apr 21, 2017 at 8:47 AM, Dennis Oleksyuk <dennis@oleksyuk.me> wrote:
Because building hardware is harder than building software. It requires more skills, time, and money. That's the main reason which applies to hardware development as a whole.

Because the number of customers who buy scientific equipment is small. Therefore the manufacturer has to divide the development cost between a smaller number of customers.

Because the cost of consumables and labor for solving a particular problem is usually higher than the cost of the machine. Therefore the buyers are more interested in saving in labor and consumable cost rather than hardware prices.


On Fri, Apr 21, 2017 at 8:42 AM Gerald Trost <gerald.trost@mail.com> wrote:

this is only my honest opinion from my experience:

I bought a open source 3d printer - in industry they had
such things for 3 decades - but they costed
several hundred thousands

the open source thing works fine but for 90% of the time
its in maintenance and I am the machine engineer

I think the open source things are not yet reliable enough.
 
Sent: Friday, April 21, 2017 at 1:57 PM
From: "Ujjwal Thaakar" <ujjwalthaakar@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
How do the economics work out and why have we not seen bigger companies bring down the process in the advent of open source equipment as well as new startups building low-cost equipment?

 

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Anyone here has an example of how results differ on different PCR machines? Like how much and on what parameter because so many researchers have told me that they don't want to disturb their setup!

On Sun, Apr 23, 2017 at 12:26 AM Gerald Trost <gerald.trost@mail.com> wrote:

Hi all!

Andreas, did you build your own thermocycler ?
I only ask because I wonder how many of us have successfully tried it.

In my chest there beat 2 hearts:

one says
"I am DIY enthusiast, I want to be able to do anything on
my own - at least I want to have the know-how"
 
the other heart says:
"I tried to build so many things and always did I end up
as machine engineer and problem solver"

I have seen OpenPCR and many similar projects on youtube
and I still think these are too expensive.

I dont think the peltier and the heat block are enough -
I suspect that rapid cooling and distributing heat
evenly might be the major issues.

Once I find the time I will bend a thin copper pipe to rings
in order to take up the reaction tubes and let some cooling
liquid run through these pipes in order to heat and to cool.
(Arduino drives peltier and a pump ...)

Maybe I am off topic because I am not really that
familiar with lab technology, if so, please let me know.

The basic idea of my design will be to spread heat and
the cooling evenly - as good as possible.

if somebody is intersted then please, suggest a
discussion forum where DIY-builders can discuss
the "Easy LiquidThermoCycler"

Gerald





 
 
Sent: Saturday, April 22, 2017 at 8:11 AM
From: "Mega [Andreas Stuermer]" <masterstorm123@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
Demand and supply. Everybody of 8 billion humans wants a smartphone and there is enourmous competition and pressure to make it as cheap as possible.

Way less demand for thermocyclers, so less companies building them. Then theres the grant system so it's not the researchers personal money and nobody says "are you stupid? I'm not gonna pay you 5000$ for a heat block and a peltier" and builds his own

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Chief Everything Officer

Kesar | Linkedin | Twitter

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive


Hi all!

Andreas, did you build your own thermocycler ?
I only ask because I wonder how many of us have successfully tried it.

In my chest there beat 2 hearts:

one says
"I am DIY enthusiast, I want to be able to do anything on
my own - at least I want to have the know-how"
 
the other heart says:
"I tried to build so many things and always did I end up
as machine engineer and problem solver"

I have seen OpenPCR and many similar projects on youtube
and I still think these are too expensive.

I dont think the peltier and the heat block are enough -
I suspect that rapid cooling and distributing heat
evenly might be the major issues.

Once I find the time I will bend a thin copper pipe to rings
in order to take up the reaction tubes and let some cooling
liquid run through these pipes in order to heat and to cool.
(Arduino drives peltier and a pump ...)

Maybe I am off topic because I am not really that
familiar with lab technology, if so, please let me know.

The basic idea of my design will be to spread heat and
the cooling evenly - as good as possible.

if somebody is intersted then please, suggest a
discussion forum where DIY-builders can discuss
the "Easy LiquidThermoCycler"

Gerald





 
 
Sent: Saturday, April 22, 2017 at 8:11 AM
From: "Mega [Andreas Stuermer]" <masterstorm123@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
Demand and supply. Everybody of 8 billion humans wants a smartphone and there is enourmous competition and pressure to make it as cheap as possible.

Way less demand for thermocyclers, so less companies building them. Then theres the grant system so it's not the researchers personal money and nobody says "are you stupid? I'm not gonna pay you 5000$ for a heat block and a peltier" and builds his own

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Re: [DIYbio] Glowing plant project fails

Classic Philosophers dilemma "The less you know, the more confident you are" you will be able to deliver ...

On Sat, Apr 22, 2017 at 3:19 AM, David Murphy <murphy.david@gmail.com> wrote:
I'm honestly surprised it failed so totally. I was under the impression that they were basically replicating earlier work that made some very faintly glowing plants.

I expected them to end up shipping something you might just about be able to see glowing in a dark room and end up stuck on the problem of getting it brighter, not utterly bombing.

On Fri, Apr 21, 2017 at 9:20 PM, Cas Smith <charles.cas.smith@gmail.com> wrote:
Kickstarter's recent 'wish list' (Our Design & Tech Team's 'Request for Projects', see: Boundary Pushers section) gives me hope that biotech/synbio/etc. will still be welcomed on their platform. They also recently hosted an event for the Biodesign Challenge. So...think positive thoughts (and advocate), I guess?



On Thursday, April 20, 2017 at 1:00:37 PM UTC-4, Cathal Garvey wrote:
I feel that this project should never have even started, because it's
implausible even on physical grounds. But, having said that.. they
could have just declared it a "fail" and walked away, at least they're
still trying to deliver something that would interest their backers.

I'm not super hopeful, but it's better than nothing. I've got no "skin
in the game" though as I never backed the project to begin with.

Whatever hopes we ever had of getting Kickstarter to stop banning
bioscience projects is slimmer now, though; the credibility is burned
and there hasn't been as much advocacy to lift the ban as there was to
instate it.

For my part, I'll still never back something on Kickstarter as long as
the ban remains. They only introduced it at the behest of the
anti-science swarm, there is no rational basis to it.

--
@onetruecathal / @cat...@quitter.is


On Thu, Apr 20, 2017 at 5:49 PM, John Griessen <jo...@industromatic.com>
wrote:
> On 04/18/2017 08:29 PM, Koeng wrote:
>> I think we all saw this coming.
>
> Oh, I didn't think they would bomb that bad -- maybe just have plants
> that faded after a few sexual reproduction cycles.
>
> $484K spent without money to ship the main reward is pretty bad
> though.
> At least they are going to lay off someone and ship something before
> the money is gone.
>
> If only they had located their project down the street from Sebastian.
> Probably would have had glowing moss graffiti last October and
> shipping glowing
> leaf plants today.
>
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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

On 04/22/2017 02:01 AM, Ujjwal Thaakar wrote:
> How do you guys see the growth of individuals who demand low cost equipment?

Probably paltry at best for the next 2 years. Who knows after that?

Best to focus on real innovation rather than "cost down" only tactics in product development.
I had a more optimistic attitude about hacker/buyer numbers and growth back
when kickstarter was fairly new and a project called makerbeam launched. Makerbeam
is smaller than usual scale struts, fasteners and brackets to assemble any prototype with.
The person doing that eventually sold it as a going business to someone in
Europe because it didn't pay his silicon valley rent.
His value add was simply batch production with standard aluminum extrusion methods,
then selling to distributors or direct, so not a ton of innovation there. Most
end user customers want more than generic parts, they want a function. It helps to
target sales to more than builders, makers, hobbyists, engineers. Everyone else
wants an "easy button" of some kind.

I'd look to the growth of individuals who demand up to the minute tech in their
lab gear -- innovation relative to the classical ways, and price in a middle ground between cheap
and "the usual" price.

And like Mega says, "> Then theres the grant system so it's not the researchers personal money and nobody says "are you stupid?
I'm not gonna pay you 5000$ for a heat block and a peltier" and builds his own"

This phenomenon of, 'nobody says "are you stupid?"', is another version of herd mentality in scientists
similar to stock market old fashioned wisdom as in, "Nobody's going to call you stupid for buying IBM."
That translates to needing some marketing BS, industrial designed housings and market share to get some of the sales
of niche market equipment like lab gear. A book called Crossing the Chasm details this product life cycle stage.
But there's still little bits of sales possible to scientists
who don't think with the herd. Be sure to sell them an "easy button" though.

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Re: [DIYbio] Glowing plant project fails

On 04/22/2017 05:19 AM, David Murphy wrote:
> I expected them to end up shipping something you might just about be able to see glowing in a dark room and end up stuck on the
> problem of getting it brighter, not utterly bombing.
>
They say they will endure pain to ship moss with a smell. So they could be close to shipping something that glows.
They might still. I bet if you study all about it you will find high rent and high pay for employees slowing/stopping them.

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Re: [DIYbio] Glowing plant project fails

I'm honestly surprised it failed so totally. I was under the impression that they were basically replicating earlier work that made some very faintly glowing plants.

I expected them to end up shipping something you might just about be able to see glowing in a dark room and end up stuck on the problem of getting it brighter, not utterly bombing.

On Fri, Apr 21, 2017 at 9:20 PM, Cas Smith <charles.cas.smith@gmail.com> wrote:
Kickstarter's recent 'wish list' (Our Design & Tech Team's 'Request for Projects', see: Boundary Pushers section) gives me hope that biotech/synbio/etc. will still be welcomed on their platform. They also recently hosted an event for the Biodesign Challenge. So...think positive thoughts (and advocate), I guess?



On Thursday, April 20, 2017 at 1:00:37 PM UTC-4, Cathal Garvey wrote:
I feel that this project should never have even started, because it's
implausible even on physical grounds. But, having said that.. they
could have just declared it a "fail" and walked away, at least they're
still trying to deliver something that would interest their backers.

I'm not super hopeful, but it's better than nothing. I've got no "skin
in the game" though as I never backed the project to begin with.

Whatever hopes we ever had of getting Kickstarter to stop banning
bioscience projects is slimmer now, though; the credibility is burned
and there hasn't been as much advocacy to lift the ban as there was to
instate it.

For my part, I'll still never back something on Kickstarter as long as
the ban remains. They only introduced it at the behest of the
anti-science swarm, there is no rational basis to it.

--
@onetruecathal / @cat...@quitter.is


On Thu, Apr 20, 2017 at 5:49 PM, John Griessen <jo...@industromatic.com>
wrote:
> On 04/18/2017 08:29 PM, Koeng wrote:
>> I think we all saw this coming.
>
> Oh, I didn't think they would bomb that bad -- maybe just have plants
> that faded after a few sexual reproduction cycles.
>
> $484K spent without money to ship the main reward is pretty bad
> though.
> At least they are going to lay off someone and ship something before
> the money is gone.
>
> If only they had located their project down the street from Sebastian.
> Probably would have had glowing moss graffiti last October and
> shipping glowing
> leaf plants today.
>
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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Completely agree with Nathan and others. A year back I was hoping the biohacking community would grow rapidly leading to an increased demand of low cost equipment but looking at OpenPCR and MiniPCR amongst others it clearly doesn't seem to be the case. It doesn't appear these companies are growing at a rapid pace. How do you guys see the growth of individuals who demand low cost equipment?
On Sat, Apr 22, 2017 at 11:42 AM Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:
Ah, Nathan beat me to it ;)

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Chief Everything Officer

Kesar | Linkedin | Twitter

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[DIYbio] Re: Gel Red questions

Now that would be a project. Masspec of gelred and hacking it DIY

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Ah, Nathan beat me to it ;)

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Demand and supply. Everybody of 8 billion humans wants a smartphone and there is enourmous competition and pressure to make it as cheap as possible.

Way less demand for thermocyclers, so less companies building them. Then theres the grant system so it's not the researchers personal money and nobody says "are you stupid? I'm not gonna pay you 5000$ for a heat block and a peltier" and builds his own

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

On 04/21/2017 10:47 AM, Dennis Oleksyuk wrote:
> Because the cost of consumables and labor for solving a particular problem is usually higher than the cost of the machine.
> Therefore the buyers are more interested in saving in labor and consumable cost

This is one place where some equipment suppliers are going slower than I think they ought to. It's where I
aim my new developments -- how to do something that has been done before with less labor and more
automated steps. I think most lab gear companies are not adapting as quickly as they should with all the nice
hardware development tools we have now. I'm working on shoestring low budget stuff, but if some of it hits, there will be no
trouble affording better plastic molds to attract some of the customers who demand flashy looks. Most lab customers will be
buying because of automation tie ins and saving steps in processes more than the housing it comes in.

To come up with time saving steps that have not been done before is not easy and that's a reason the
equipment costs -- to pay for the "not easy" design steps. Established companies have a lot of designed "looks"
to keep up and that could be making them hold back on introducing new designs as quickly as they could.
The plastic molds for those "looks" are very expensive, so that is a hurdle to action.

I'm going to be trying out designing/buying 3D printed molds to use to make product, and the molds won't be up to snuff for
cell phone types of products, only maybe forgivable as ways to get housings for scientist tools to test
low volume equipment ideas. Another way to get a product out is to carve housings out of plastic
instead of molding anything -- plastic molds can take a lot of time, cost a ton, weigh a ton.
The culture shock electroporator project will have carved then molded housings as it progresses.
The molds will be light weight aluminum things that won't last for volume production, just to get started
and be able to change and adapt.

PCR machines with vials are definitely needing lots of molding tech, so the above expense reasons fit them.
--
John Griessen

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Re: [DIYbio] Glowing plant project fails

Kickstarter's recent 'wish list' (Our Design & Tech Team's 'Request for Projects', see: Boundary Pushers section) gives me hope that biotech/synbio/etc. will still be welcomed on their platform. They also recently hosted an event for the Biodesign Challenge. So...think positive thoughts (and advocate), I guess?



On Thursday, April 20, 2017 at 1:00:37 PM UTC-4, Cathal Garvey wrote:
I feel that this project should never have even started, because it's
implausible even on physical grounds. But, having said that.. they
could have just declared it a "fail" and walked away, at least they're
still trying to deliver something that would interest their backers.

I'm not super hopeful, but it's better than nothing. I've got no "skin
in the game" though as I never backed the project to begin with.

Whatever hopes we ever had of getting Kickstarter to stop banning
bioscience projects is slimmer now, though; the credibility is burned
and there hasn't been as much advocacy to lift the ban as there was to
instate it.

For my part, I'll still never back something on Kickstarter as long as
the ban remains. They only introduced it at the behest of the
anti-science swarm, there is no rational basis to it.

--
@onetruecathal / @cat...@quitter.is


On Thu, Apr 20, 2017 at 5:49 PM, John Griessen <jo...@industromatic.com>
wrote:
> On 04/18/2017 08:29 PM, Koeng wrote:
>> I think we all saw this coming.
>
> Oh, I didn't think they would bomb that bad -- maybe just have plants
> that faded after a few sexual reproduction cycles.
>
> $484K spent without money to ship the main reward is pretty bad
> though.
> At least they are going to lay off someone and ship something before
> the money is gone.
>
> If only they had located their project down the street from Sebastian.
> Probably would have had glowing moss graffiti last October and
> shipping glowing
> leaf plants today.
>
> --
> -- You received this message because you are subscribed to the Google
> Groups DIYbio group. To post to this group, send email to
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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

I agree with the previous folks. In simplest terms it comes down to
the economics 'law' of supply and demand.

Take a piece of hardware like a smartphone. There is so much quality
control that you probably don't think about, it is insanely expensive
and time consuming. Who hasn't experienced their smartphone doing
something dumb or buggy? While it annoys phone users, it usually isn't
a major loss of money. In science that could ruin an experiment (which
are often very very expensive), so end-users are a lot more cautious
and willing to pay for products that are as bug-free as possible. This
also means that less well-tested products will have a harder time
gaining market share, scientists are less swayed by flashy marketing
(less, not completely immune) than the average hardware consumer
because, well just look at all the people being scammed left and right
with TV infomercial products, kickstarters, etc.

On Fri, Apr 21, 2017 at 8:47 AM, Dennis Oleksyuk <dennis@oleksyuk.me> wrote:
> Because building hardware is harder than building software. It requires more
> skills, time, and money. That's the main reason which applies to hardware
> development as a whole.
>
> Because the number of customers who buy scientific equipment is small.
> Therefore the manufacturer has to divide the development cost between a
> smaller number of customers.
>
> Because the cost of consumables and labor for solving a particular problem
> is usually higher than the cost of the machine. Therefore the buyers are
> more interested in saving in labor and consumable cost rather than hardware
> prices.
>
>
> On Fri, Apr 21, 2017 at 8:42 AM Gerald Trost <gerald.trost@mail.com> wrote:
>>
>>
>> this is only my honest opinion from my experience:
>>
>> I bought a open source 3d printer - in industry they had
>> such things for 3 decades - but they costed
>> several hundred thousands
>>
>> the open source thing works fine but for 90% of the time
>> its in maintenance and I am the machine engineer
>>
>> I think the open source things are not yet reliable enough.
>>
>> Sent: Friday, April 21, 2017 at 1:57 PM
>> From: "Ujjwal Thaakar" <ujjwalthaakar@gmail.com>
>> To: DIYbio <diybio@googlegroups.com>
>> Subject: [DIYbio] Why are PCR machines and biotech equipment in general so
>> expensive
>> How do the economics work out and why have we not seen bigger companies
>> bring down the process in the advent of open source equipment as well as new
>> startups building low-cost equipment?
>>



--
-Nathan

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive

Because building hardware is harder than building software. It requires more skills, time, and money. That's the main reason which applies to hardware development as a whole.

Because the number of customers who buy scientific equipment is small. Therefore the manufacturer has to divide the development cost between a smaller number of customers.

Because the cost of consumables and labor for solving a particular problem is usually higher than the cost of the machine. Therefore the buyers are more interested in saving in labor and consumable cost rather than hardware prices.


On Fri, Apr 21, 2017 at 8:42 AM Gerald Trost <gerald.trost@mail.com> wrote:

this is only my honest opinion from my experience:

I bought a open source 3d printer - in industry they had
such things for 3 decades - but they costed
several hundred thousands

the open source thing works fine but for 90% of the time
its in maintenance and I am the machine engineer

I think the open source things are not yet reliable enough.
 
Sent: Friday, April 21, 2017 at 1:57 PM
From: "Ujjwal Thaakar" <ujjwalthaakar@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
How do the economics work out and why have we not seen bigger companies bring down the process in the advent of open source equipment as well as new startups building low-cost equipment?

 

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Re: [DIYbio] Why are PCR machines and biotech equipment in general so expensive


this is only my honest opinion from my experience:

I bought a open source 3d printer - in industry they had
such things for 3 decades - but they costed
several hundred thousands

the open source thing works fine but for 90% of the time
its in maintenance and I am the machine engineer

I think the open source things are not yet reliable enough.
 
Sent: Friday, April 21, 2017 at 1:57 PM
From: "Ujjwal Thaakar" <ujjwalthaakar@gmail.com>
To: DIYbio <diybio@googlegroups.com>
Subject: [DIYbio] Why are PCR machines and biotech equipment in general so expensive
How do the economics work out and why have we not seen bigger companies bring down the process in the advent of open source equipment as well as new startups building low-cost equipment?

 

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[DIYbio] Why are PCR machines and biotech equipment in general so expensive

How do the economics work out and why have we not seen bigger companies bring down the process in the advent of open source equipment as well as new startups building low-cost equipment?

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[DIYbio] Why are PCR machines and biotech equipment in general so expensive

How do the economics work out and why have we not seen bigger companies bring down the process in the advent of open source equipment as well as new startups building low cost equipment?
--

Ujjwal Thaakar

Chief Everything Officer

Kesar | Linkedin | Twitter

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Re: [DIYbio] Glowing plant project fails

I feel that this project should never have even started, because it's
implausible even on physical grounds. But, having said that.. they
could have just declared it a "fail" and walked away, at least they're
still trying to deliver something that would interest their backers.

I'm not super hopeful, but it's better than nothing. I've got no "skin
in the game" though as I never backed the project to begin with.

Whatever hopes we ever had of getting Kickstarter to stop banning
bioscience projects is slimmer now, though; the credibility is burned
and there hasn't been as much advocacy to lift the ban as there was to
instate it.

For my part, I'll still never back something on Kickstarter as long as
the ban remains. They only introduced it at the behest of the
anti-science swarm, there is no rational basis to it.

--
@onetruecathal / @cathal@quitter.is


On Thu, Apr 20, 2017 at 5:49 PM, John Griessen <john@industromatic.com>
wrote:
> On 04/18/2017 08:29 PM, Koeng wrote:
>> I think we all saw this coming.
>
> Oh, I didn't think they would bomb that bad -- maybe just have plants
> that faded after a few sexual reproduction cycles.
>
> $484K spent without money to ship the main reward is pretty bad
> though.
> At least they are going to lay off someone and ship something before
> the money is gone.
>
> If only they had located their project down the street from Sebastian.
> Probably would have had glowing moss graffiti last October and
> shipping glowing
> leaf plants today.
>
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Re: [DIYbio] Glowing plant project fails

On 04/18/2017 08:29 PM, Koeng wrote:
> I think we all saw this coming.

Oh, I didn't think they would bomb that bad -- maybe just have plants
that faded after a few sexual reproduction cycles.

$484K spent without money to ship the main reward is pretty bad though.
At least they are going to lay off someone and ship something before the money is gone.

If only they had located their project down the street from Sebastian.
Probably would have had glowing moss graffiti last October and shipping glowing
leaf plants today.

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Position : Splunk Consultant -- Manassas, VA

Hi,

 

Please find the below job description and respond back to me with your consultants profiles to praveen@sritechsolutions.com

 

Position : Splunk Consultant

Location : Manassas, VA

Duration : 6 + months Contract

 

We are seeking a Splunk Consultant with ability to support, design, and develop enterprise systems data management. The qualified candidate will be responsible for support, development, integration and management of Splunk in addition to a variety of other tasks.
 
Responsibilities:

·         Candidate will drive deployments of Splunk while working side by side with the customers to solve their unique problems across a variety of use cases

·         Candidate will deploy ITSI and build service models for alignemnt with customer facing applications and networks

·         Candidate will drive standardization of data collection across devices within the organization

·         Develop and deploy unique collectors using scripts and collectors developed

·         Collaborate across the entire organization to bring Splunk access to product and technical teams to get the right solution delivered and drive future innovation gathered from customer input

·         Leverage previous experience, share best practice and create solutions to push user adoption and maximize the value of Splunk

·         Design, Deploy, support and maintain Splunk cluster infrastructure in a highly available, geo-redundant configuration

·         Standardized Splunk agent deployment, configuration and maintenance across a variety of platforms using multiple configuration management systems

·         Troubleshoot Splunk server and agent problems and issues

·         Assist internal users of Splunk in designing and maintaining production-quality dashboards

·         Participate as a Splunk escalation point for operations support

·         Monitor the agent and server infrastructure for capacity planning and optimization 

·         Design core Systems performance and troubleshooting logs

·         Support Splunk on Unix, Linux and Windows-based platforms

 
Basic Requirements:

·         2-4 years of experience with data analysis, logging solutions, system and network monitoring, and technical consulting

·         Ability to assess customer's situation, business needs, complex problems, and provide a solution forward

·         Experience with both the Unix and Windows operating systems; comfortable on the command line interface

·         Working knowledge or recent experience with scripting languages (i.e., Bash or Perl) or application development (JAVA, Python, .NET), relational databases, and analytical tools

·         Ability to stop, collaborate and listen with technical and non-technical consumers from IT administrators to executive level stakeholders

·         Self-motivated and self-educating, able to function independently as needed.  Also, need to be able to collaborate with fellow Splunk engineers as well as customers. 

·         Ability to function independently and in a team is a MUST.

·         Well organized with a healthy sense of urgency, able to set; communicate; and meet aggressive deadlines with competing priorities

·         Demonstrable understanding of common enterprise applications (especially in the areas of security and finance)

 
Preferred:

·         Splunk Certification

·         Previous training and hands on experience with Splunk

·         Operational experience with any of the following areas:  network engineering, Linux/Unix systems administration, Windows/Active Directory administration, network security, firewalls, NIDS, NIPS, web proxy solutions, email security, systems security (HIPS/HIDS), security analyst, storage, virtualization, mail servers, data modeling and pivot tables

·         Experience with Virtualization technologies

·         Experience in one of the following programming languages:  Java, C++, Python, Ruby, Perl

·         Experience with Regular Expressions (REGEX)

·         Previous experience with a software configuration management system (subversion, git, hub, cvs, chef, puppet, SCCM, etc.)

·         Microsoft, Linux or networking certifications

 
Desirable:

·         Strong oral and written communication skills: including documentation

·         Ability to work collaboratively and lead within Splunk analytics team; ability to work independently as well

·         Ability to handle multiple tasks concurrently

·         Strong interpersonal and leadership skills

·         Good in interaction with business and technology

·         Ability to work with customer stakeholders and define their needs and translate those needs into Splunk queries and dashboards

·         Statistical and analytical modeling or Business Intelligence

 

-

 

Thanks and Best Regards,

 

Praveen Kumar

SRI Tech Solutions Inc.

praveen@sritechsolutions.com

(T) 1-813-423-6500 X Ext: 149 (M) 248-462 -7719 (F) 813.423.6520

 

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